Fig. 2

HE4 protein expression levels in control (Ctrl), HE4 knockdown with siRNA (siHE4), Pac treatment (Pac), and knockdown plus Pac treatment (siHE4 + Pac) groups. Western blotting was performed with HE4- and GAPDH-specific antibodies, respectively, following cell transfection with HE4 expression plasmid DNA or Pac treatment. a Representative results of Western blot analysis for HE4 and GAPDH. b The results were scanned for densitometry analysis. Levels of HE4 were normalized with those of GAPDH. A nearly 60% reduction in HE4 level was achieved by siRNA-mediated knockdown. HE4 levels remained stable following Pac treatment