Fig. 3
From: Biological effects of selective COX-2 inhibitor NS398 on human glioblastoma cell lines
NS398 strongly affects GBM-derived neurospheres’ growth, without influencing cell viability. a, b Neurosphere-derived cell viability evaluated by flow cytometric analysis with PI staining. The percent viable and dead cells are reported in the histograms. c Representative phase-contrast images (4× magnification) of U87MG neurospheres in the absence (DMSO-treated, CNTR) or presence of COX-2 activity inhibitor NS398 (100 μM for 48 h). The quantitative analysis of neurospheres’ mean area was expressed as percentage vs relative T0. d Representative phase-contrast images (4× magnification) of T98G neurospheres in the absence (CNTR) or presence of COX-2 activity inhibitor NS398 (100 μM for 48 h). The quantitative analysis of neurospheres’ mean area was expressed as percentage vs relative T0. The results are representative of two independent experiments and are expressed as mean values of duplicates ± SEM. For comparison between two means, Student’s unpaired t-test was used (*p < 0.05)