Fig. 3From: N-alkylisatin-based microtubule destabilizers bind to the colchicine site on tubulin and retain efficacy in drug resistant acute lymphoblastic leukemia cell lines with less in vitro neurotoxicityLevels of intracellular rhodamine 123 (R123) in CEM and CEM-VCR R cell lines in the presence and absence of cyclosporine A (CSA). a Parental (CEM) and VCR-resistant cells (CEM-VCR R) were incubated with R123 for 20 min before being washed with PBS and lysed with 1% Triton-X. b CEM-VCR cells were incubated with R123 in the presence or absence of 20 µM Cyclosporin A (CSA), a specific P-gp inhibitor, for 20 min before undergoing subsequent washing and lysis. Intracellular fluorescence was subsequently measured using a FLUOstar Optima Microplate Reader using an excitation wavelength of 485 nm, and emission wavelength of 530 nm. Mean ± SD, n = 3. ***P < 0.001, ****P < 0.0001Back to article page