Fig. 4
From: High expression of ESRP1 regulated by circ-0005585 promotes cell colonization in ovarian cancer
ESRP1 alternative spliced cytoskeleton to promote MET process. a, b Phalloidin staining was used to dye F-actin to display morphological changes in ESRP1 overexpression EOC cells (a) and circ-0005585 transfected SKOV3 and A2780 cells (b). Phalloidin staining (red) indicated F-actin. DAPI staining (blue) indicated nucleuses. Scale bar 10 μm. c GO enrichment terms of differential genes in ESRP1-SKOV3 cells compared with EV-SKOV3. d ESRP1 alternative splicing differential genes were mainly enriched in two GO terms: actin cytoskeleton organization and epithelial cell morphogenesis. e ESRP1 regulated the subtypes of the cytoskeleton-associated protein EPB41L5 by alternative splicing the end exon, validated in ESRP1-SKOV3 and ESRP1-A2780 by qRT-PCR. f ESRP1 regulated cytoskeleton-associated protein Rac1 by exon skipping and verified by qRT-PCR in the SKOV3 and A2780 cells. g Transfection of circ-0005585 up-regulated ESRP1 and promoted alternative splicing EPB41L5 in ESRP1-SKOV3 and ESRP1-A2780. *P<0.05, **P<0.01, ***P<0.001. Error bars indicate SEM