Fig. 3

H3K27me3 contributes to FOSB transcription silencing in TNBC. a The mRNAs of FOSB in MDA-MB-231 and MDA-MB-436 cells with or without 10 μM 5-Aza-CdR treatment were determined by real-time PCR. b Immunoblot analysis of the protein levels of FOSB in (a). c ChIP shows enrichment of H3K27me3 at the FOSB promoter in MDA-MB-231 cells. d Immunoblot analysis of the H3K27me3 and H3K27Ac protein levels in MDA-MB-231 cells with or without 2 μM GSK343 treatment. e ChIP assay shows treatment with 2 μM GSK343 in MDA-MB-231 cells depleted H3K27me3 and increased H3K27ac from the FOSB promoter