Fig. 2

Effect of TNFAIP8 depletion on proliferation, migration and invasion of breast cancer cells. a Inhibition of TNFAIP8 reduced the expression and protein levels of TNFAIP8 in BT-459 and MDA-MB-231 cells detected by qRT-PCR and Western blotting analyses. b Inhibition of TNFAIP8 inhibited proliferation of BT-459 and MDA-MB-231 cells detected by CCK-8 assay. c Inhibition of TNFAIP8 reduced clonogenicity of BT-459 and MDA-MB-231 cells detected by colony formation assay. d–e Inhibition of TNFAIP8 repressed migration and invasion of BT-459 and MDA-MB-231 cells detected by Transwell assay. f Protein levels of N-cadherin, Vimentin and Snail, and E-cadherin in BT-459 and MDA-MB-231 cells with treatment of TNFAIP8-siRNA detected by Western blotting analysis. Measurements were carried out in triplicate, and experiments were repeated three times. Data are presented as mean ± SD. * p < 0.05 and ** p < 0.01