Fig. 3

Knockdown of Nanos3 inhibits glioblastoma cell growth and migration in vitro. a A CCK-8 cell proliferation assay was performed after Nanos3 deletion in A172, U251, and LN229 cells. b An alkaline phosphatase stain assay was performed between the WT GBM cell lines and the Nanos3 deletion cells. Images were taken from the inverted microscope (bars = 50 μm; magnification ×200). c The statistical analysis of the positive ALP staining of the glioblastoma cells. d Western blot analysis determined Cyclin D1 protein expression in GBM cell lines and the nanos3 KD cells. The statistical analysis of the protein expression in the right pane. e Cell migration assays were performed after Nanos3 deletion in A172, U251, and LN229 cells. A172, U251, and LN229 cells with Nanos3 knockdown exhibited decreased ability to migrate through the Boyden chamber compared with the WT GMB cell lines (bars = 50 μm; magnification ×200). f The statistical analysis of the ability of the glioma cells’ migration. g The statistical analysis of the wound-healing assay. The wound-healing assay was performed between GBM WT cells and Nanos3 knockdown cells. All experiments were carried out in triplicate. All data are shown as the mean ± SE. *P < 0.01, **P < 0.01, ***P < 0.001