Fig. 4

Knockdown of Nanos3 inhibits glioblastoma cell chemoresistance and invasion in vitro. a Nanos3 KD cells were significantly more sensitive to DOX and TMZ than GBM WT cells (A172, U251, and LN229). Viable cells were stained with crystal violet, images were taken with an inverted microscope (bars = 50 μm; magnification ×100). b The statistical analysis of the glioma cell resistance. All experiments were carried out in triplicate. Data are shown as the mean ± SE. ^#,*P < 0.05; ^##,**P < 0.01; ^###,***P < 0.001. c A matrigel cell invasion assay was performed after the deletion of Nanos3 in GBM cell lines. Nanos3^+/− cells showed a decreased ability to invade through the Matrigel chamber compared with the Nanos3 WT GMB cell lines. Cells were stained with crystal violet, images were taken using an inverted microscope (magnification ×100). d The statistical analysis of the glioma cell invasion. All experiments were carried out in triplicate. Data are shown as the mean ± SE. ***P < 0.001