Fig. 5

Knockdown of NNT-AS1 attenuates DDP resistance in DDP-resistant CC cells through regulating miR-186. HeLa/DDP and SiHa/DDP cells were transfected with sh-NC, sh-NNT-AS1, sh-NNT-AS1 + anti-miR-NC or sh-NNT-AS1 + anti-miR-186. a–c Cell viability and proliferation of HeLa/DDP and SiHa/DDP cells were detected by MTT assay. d The apoptosis rates were analyzed by Flow Cytometry in HeLa/DDP and SiHa/DDP cells. e, f The protein expression of cleaved caspase-3, Bcl-2 and Bax in HeLa/DDP and SiHa/DDP cells was detected by western blot. g, h Transwell assay was used to analyze migration and invasion abilities of HeLa/DDP and SiHa/DDP cells. i, j The levels of E-cadherin N-cadherin and Vimentin were detected by western blot. *P < 0.05