Fig. 6

HMGB1 is a target of miR-186 and NNT-AS1 can regulate HMGB1 expression via targeting miR-186. a–c The expression of HMGB1 in DDP-sensitive or DDP-resistance CC tissues and cell lines was analyzed by qRT-PCR or western blot, respectively. d The correlation between HMGB1and miR-186 was determined. e The correlation between HMGB1and NNT-AS1 was analyzed. f The predicted binding sequences between HMGB1 and miR-186 were exhibited. g, h Luciferase reporter assay was conducted to evaluate the luciferase activity in HeLa/DDP and SiHa/DDP cells after the co-transfected with HMGB1-WT or HMGB1-MUT and miR-186 mimic or miR-NC. i–l QRT-PCR or western blot was used to analyze the expression of HMGB1 in HeLa/DDP and SiHa/DDP cells transfected with miR-NC or miR-186 or miR-186 and pcDNA or miR-186 and NNT-AS1. *P < 0.05