Fig. 2

Knock down of LINC00689 restrains prostate cancer progression. a The knockdown efficiency of LINC00689 was detected in DU145 and LNCaP cells after 48 h of transfection with sh-LINC00689#1/#2. b, c MTT (detected after incubated for indicated time) and colony formation (measured after incubated for 14 days) assays measured the effects of knocking down LINC00689#1/#2 on DU145 and LNCaP cell proliferation. d Flow cytometry analysis measured the apoptosis in LINC00689 silenced DU145 and LNCaP cells. e Western blot assay examined that protein level of cleaved caspase 3 in sh-LINC00689#1 transfected DU145 and LNCaP cells. GAPDH was an internal control. f Caspase-3 test displayed the caspase-3 activity in response to LINC00689 downregulation in DU145 and LNCaP cells. g, h Transwell assay determined the effects of LINC00689 silencing on DU145 and LNCaP cell migration and invasion after incubated for 24 h. Images were captured by using the inverted microscope (4 × objective lens) (scale bar = 100 μm). Error bars represent the mean ± SD of at least three independent experiments. ^**P < 0.01