Fig. 6

LINC00689 promotes prostate cancer progression by overexpressing CTNNB1. a, b MTT (cultured for 0, 1, 2, 3, 4 days) and colony formation (after 14 days of incubation) assays detected DU145 cell proliferation in differently transfected groups (sh-NC, sh-LINC00689#1 and sh-LINC00689#1 + pcDNA3.1/CTNNB1). c Flow cytometry measured DU145 cell apoptosis in differently transfected groups (sh-NC, sh-LINC00689#1 and sh-LINC00689#1 + pcDNA3.1/CTNNB1). d Western blot assay measured cleaved caspase 3 expression in differently transfected groups (sh-NC, sh-LINC00689#1 and sh-LINC00689#1 + pcDNA3.1/CTNNB1). GAPDH was an internal control. e Caspase 3 activity was measured in DU145 cell proliferation in differently transfected groups (sh-NC, sh-LINC00689#1 and sh-LINC00689#1 + pcDNA3.1/CTNNB1). f, g Transwell assay (incubated for 24 h) measured DU145 cell migration and invasion in differently transfected groups (sh-NC, sh-LINC00689#1 and sh-LINC00689#1 + pcDNA3.1/CTNNB1). Images were captured by using the inverted microscope (4 × objective lens) (scale bar = 100 μm). Error bars represent the mean ± SD of at least three independent experiments. ^**P < 0.01