Fig. 3
From: Oncogenic role of HMGA2 in fusion-negative rhabdomyosarcoma cells
Derivation of myogenic differentiation by HMGA2 siRNA knockdown. a Representative light microscopy images of HMGA2-depleted RD cells and control RD cells after 72 h in differentiation medium. Scale bar, 100 mm. Some HMGA2-depleted RD cells formed myotubes. b Fluorescent images of MHC staining after 72 h in differentiation medium. Scale bar, 50 mm. Representative images of HMGA2-depleted RD cells showing MHC and DAPI (for nuclei), whereas control RD cells showed few MHC-positive cells. c Numbers of MHC-positive cells per 5.0 × 103 cells. Results represent the mean ± SD of three independent experiments. *P < 0.01 compared with control RD cells