Skip to main content
Fig. 3 | Cancer Cell International

Fig. 3

From: HMGB1 mediates lipopolysaccharide-induced inflammation via interacting with GPX4 in colon cancer cells

Fig. 3

HMGB1 interacts with GPX4. a The cells were transfected with siHMGB1 to observe the changes of GPX4 activity, and the results showed that siHMGB1 could increase the GPX4 activity which was repressed by LPS. b IP results indicated that HMGB1 interacted with GPX4 in a dose-dependant manner with LPS simulated, but cells without LPS treatment failed to observe this interaction. c Over-expression vectors of GPX4 were constructed to be injected into SW480 and HCT116 cells. Western blot results showed that GPX4 protein expressions were up-regulated in pcDNA3.1-GPX4 groups. To investigate the function of GPX4, we detected the ROS and MDA levels and the results indicated that GPX4 could suppress the ROS accumulation (d) and MDA levels (e), which were elevated by LPS. MDA, malondialdehyde

Back to article page

Cancer Cell International

ISSN: 1475-2867

Contact us