Fig. 3

Apatinib suppressed the migration and invasion of esophageal cancer cells in vitro. a KYSE30 and TE1 cells treated with apatinib (3, 10 and 30 μM) for 24 h were scraped and imaged immediately (0 h) and later (24 h), and images of the wound gap were taken. Scale bar, 200 μm. b Quantitative analysis of the wound healing rate. P values were determined by one-way ANOVA with Tukey’s correction. *P < 0.05, **P < 0.01. c After apatinib (10 μM) treatment for 36 h, invaded cells were stained and counted using microscopy. Scale bar, 50 μm. d Quantitative analysis of the number of invaded cells. P values were determined by Student’s t test. *P < 0.05. KYSE30 and TE1 cells were treated with apatinib (10 μM) for 24 h. e The mRNA expression levels of E-cadherin, Vimentin and N-cadherin were determined by qPCR. P values were determined by Student’s t test. *P < 0.05, **P < 0.01. f The protein levels of E-Cadherin, Vimentin and N-cadherin were determined by western blotting. Data are representative of three independent experiments (mean and SEM in b, d, e)