Fig. 5

SNHG16 indirectly regulates PLK4 expression by interacting with miR-338-3p. a The putative binding site between PLK4 and miR-338-3p was listed. b–c The interaction between PLK4 and miR-338-3p was confirmed by dual-luciferase reporter assay in SK-N-AS-R and SK-N-SH-R cells. d, e The expression of PLK4 was detected using qRT-PCR in cisplatin-resistant neuroblastoma tissues and cell lines (N = 3). f, g The correlation between PLK4 and miR-338-3p or SNHG16 was analyzed using Spearman’s correlation test. h The protein expression of PLK4 in SK-N-AS-R and SK-N-SH-R cells transfected with miR-NC, miR-338-3p was measured using western blot (N = 3). i The level of PLK4 protein was examined by western blot in SK-N-AS-R and SK-N-SH-R cells transfected with pcDNA, SNHG16, SNHG16 + miR-338-3p, or SNHG16 + miR-NC (N = 3). N means that triplicate individual experiments were performed, and the average was taken. *P < 0.05