Fig. 2

Matrine suppressed the development of HCC cells by decreasing circ_0027345. a Huh-7 and HCCLM3 cells were treated with various concentrations of matrine, and circ_0027345 expression was measured by qRT-PCR analysis. b Huh-7 and HCCLM3 cells were exposed to different treatments and were divided into the following four groups: control, Matrine (Mat), Mat + pcDNA-NC and Mat + pcDNA-circ_0027345, circ_0027345 expression was then detected by qRT-PCR. c, d Cell viability and apoptosis were assessed by CCK-8 assay and Flow cytometry, respectively. e, f The levels of Bcl-2, Bax and cleaved-caspase 9 were examined by western blot. g, j Cell cycle, migration and invasion were determined by Flow cytometry and Transwell assay, respectively. k, l LC3-II, LC3-I and Beclin 1 levels were measured by western blot. *P < 0.05