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Fig. 5 | Cancer Cell International

Fig. 5

From: GSK-J4 induces cell cycle arrest and apoptosis via ER stress and the synergism between GSK-J4 and decitabine in acute myeloid leukemia KG-1a cells

Fig. 5

GSK-J4 induces cell apoptosis via ER stress. a After co-treatment with 4-PBA (3 mM) and GSK-J4 (4 μM), the ratio of apoptotic cells was analyzed using flow cytometry. b Statistical analysis of the apoptotic rate. Values represent the mean ± SD of three independent experiments.*p < 0.05, **p < 0.01. c After pre-treatment with 3 mM 4-PBA, the expression levels of bax and cleaved-caspase9 were detected by Western blotting. d, e Statistical analysis of the expression levels of Bax and cle-caspase9. β-Actin was used as an internal control. Values represent the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01

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