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Fig. 6 | Cancer Cell International

Fig. 6

From: GSK-J4 induces cell cycle arrest and apoptosis via ER stress and the synergism between GSK-J4 and decitabine in acute myeloid leukemia KG-1a cells

Fig. 6

The role of ER stress in GSK-J4-regulated PKC-α/p-Bcl2 pathway inhibition. a After treatment with PKC-α siRNA NC, siRNA1, siRNA2 and siRNA3, the expression level of PKC-α was detected by Western blotting. b After treated with siRNA2, the ratio of apoptotic cells was detected using flow cytometry. c Statistical analysis of the apoptotic rate of KG-1a treated with siRNA2. Values represent the mean ± SD of three independent experiments. *p < 0.05, ***p < 0.001. d The mRNA expression level of PKC-α was detected using qRT-PCR. GAPDH was used as an internal control. Values represent the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01. e After treatment with GSK-J4, the expression levels of PKC-α and p-Bcl2 in KG-1a cells were detected by Western blotting. f Statistical analysis of the expression levels of PKC-α and p-Bcl2. β-Actin was used as an internal control. Values represent the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01. g After pre-treatment with 4-PBA (3 mM), the expression levels of PKC-α and p-Bcl2 were detected by Western blotting. h, i Statistical analysis of the expression levels of PKC-α and p-Bcl2. β-Actin was used as an internal control. Values represent the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01

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