Fig. 2

miR-454 overexpression promotes apoptosis of ovarian cancer cells and inhibits the Akt/mTOR and Wnt/β-catenin signaling pathways. a Apoptosis rate of OVCAR3 and SKOV3 cells transfected with either pCMV-MIR-miR-454 or pCMV-MIR plasmid was measured by flow cytometry assay. b Following transfection with either pCMV-MIR-miR-454 or pCMV-MIR plasmid for 48 h, the expression of apoptosis-related proteins (Bcl-2, Bax, cleaved Caspase 9, and cleaved Caspase 3) in OVCAR3 and SKOV3 cells was detected using western blot. c, d Quantitative analysis of Western blot results in b. e Expression of the Akt/mTOR signaling pathway related proteins in OVCAR3 and SKOV3 cells was detected using western blot. f, g Quantitative analysis of Western blot results in e. h Expression of the Wnt/β-catenin signaling pathway related proteins in OVCAR3 and SKOV3 cells was detected using western blot. i, j Quantitative analysis of Western blot results in h. *P < 0.05, **P < 0.01 vs. NC group