Fig. 5
From: miR-454 suppresses the proliferation and invasion of ovarian cancer by targeting E2F6
miR-454 overexpression abolishes the effects of E2F6 on the proliferation and migration of ovarian cancer cells. a Relative expression of E2F6 mRNA in OVCAR3 and SKOV3 cells co-transfected with pcDNA3.1-E2F6 and pCMV-MIR-miR-454 or transfected with pcDNA3.1-E2F6 alone was measured by RT-PCR, cells transfected with pcDNA3.1 vector was used as negative control (NC). b, c CCK8 assay was performed to assess the proliferation of OVCAR3 (b) and SKOV3 (c) cells after indicated transfection. d Migration ability of OVCAR3 and SKOV3 cells after indicated transfection. *P < 0.05, **P < 0.01 vs. NC group, ΔP < 0.05 vs. E2F6 group