Fig. 3

ZKSCAN3 promotes epithelial-to-mesenchymal transition in HCC cell. The overexpression of ZKSCAN3 in Hep3B cells reduced the expression of the epithelial cell marker E-cadherin and increased the expression of the mesenchymal cell marker N-cadherin and Vimentin determined by western blot (a) and IF (c). In contrast, ZKSCAN3 knockdown increased E-cadherin expression and reduced N-cadherin and Vimentin expression (b, d). Immunohistochemical analysis of E-cadherin, N-cadherin and Vimentin in HCC samples. In cases of high ZKSCAN3 expression; there was low detectable E-cadherin (left, up) and strong N-cadherin, Vimentin protein (middle, right, up) expression in tissue section. In contrast, in the case of low ZKSCAN3 expression, there was strong E-cadherin (left, down) and low N-cadherin, Vimentin protein (middle, right, down) expression. Values are depicted as Mean ± SD; *P < 0.05