Fig. 7

Circ_0000284 generated the oncogenic function in NSCLC cells via the regulation of miR-377-3p/PD-L1 axis. a PD-L1 expression was assayed through western blot in A549 and H82 cells transfected with si-NC or si-PD-L1. b The examination of miR-377-3p was conducted using qRT-PCR after transfection of circ_0000284, circ_0000284 + miR-377-3p or relative controls. b Western blot was applied for measuring the protein level of PD-L1 following the transfection of circ_0000284, circ_0000284 + miR-377-3p, circ_0000284 + si-PD-L1 as well as their controls. d, e CCK-8 and colony formation assays were used for the respective detection of cell proliferation (d) and colony formation ability (e). f, g Cell migration (f) and invasion (g) were evaluated using transwell assay. h The determination of proliferation and metastasis-associated protein levels was performed via western blot. All experiments were independently performed three times with N value = 3. *P < 0.05