Fig. 3

Integrin distribution, amounts, and disassembly. a Distribution of integrin in four different cells observed by optical (left column) and fluorescence (right column) microscopy. b Western blot analysis of integrin, and the ratio of integrin to β-actin in four cells. c Morphological change of cells induced by trypsin–EDTA treatment. The same position on the cell was indicated with an arrow to compare the morphological change according to the treatment time. d Relative cell area reduction as a function of trypsin–EDTA treatment time. Each value represents mean ± SEM (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001)