Fig. 5

miR-363 inhibits the proliferation, migration and invasion of ccRCC cells by downregulating S1PR1. a Folds change of miR-363 and S1PR1 mRNA levels in 786O cells transfected with miR-363 mimic (versus NC mimics) and lentiviral S1PR1 plasmids (versus empty vector). b Folds change of miR-363 and S1PR1 mRNA levels in 769P cells transfected with miR-363 inhibitor (versus NC inhibitor) and siS1PR1 (versus siNC). c MTS assay suggested that overexpression of S1PR1 reversed the negative proliferative effects of miR-363 mimics in 786O cells. d Knockdown of S1PR1 counteracted the positive proliferative effects of miR-363 inhibitor in 769P cells. e Transwell assays suggested that overexpression of S1PR1 reversed the negative migration and invasion effects of miR-363 mimics in 786O cells. f Knockdown of S1PR1 counteracted the positive migration and invasion effects of miR-363 inhibitor in 769P cells. g Alterations of S1PR1, ERK and downstream genes of ERK protein level in 786O cells transfected with miR-363 mimic (versus NC mimics) and lentiviral S1PR1 plasmids (versus empty vector). h Alterations of S1PR1, ERK and downstream genes of ERK protein level in 769P cells transfected with miR-363 inhibitor (versus NC inhibitor) and siS1PR1 (versus siNC). Data are presented as the mean ± SD. (*P < 0.05, **P < 0.01, ***P < 0.001)