Fig. 5
TRIM2 regulates Snail1 expression through ubiquitination. a Correlation analysis of TRIM2 mRNA and Snail1 mRNA in GEPIA database. b Correlation analysis of TRIM2 mRNA and Snail1 mRNA in clinical samples. c Western blot analysis of Snail1 protein expression in TRIM2 knockdown treated A549 cells and TRIM2 overexpression treated H322 cells. d qRT-PCR analysis of Snail1 protein expression in TRIM2 knockdown treated A549 cells and TRIM2 overexpression treated H322 cells. e Correlation analysis of TRIM2 protein expression and Snail1 protein expression in clinical samples. f Western blot analysis of TRIM2 and Snail1 expression in TRIM2 over-expression treated H322 cells with CQ and MG132 treatment on different time points. g CO-IP analysis of TRIM2 and Snail1 interaction in knockdown treated A549 cells. h Forward and reverse CO-IP analysis of TRIM2 and Snail1 in H322 cells and A549 cells. i Immunocolocalization analysis of TRIM2 and Snail1 in H322 cells and A549 cells. j, k Rrmaining Snail1 protein in H322 cells and A549 cells with cyclohexane (CHX) treatment on different time points. l, m Ubiquitination test of TRIM2 and Snail1 in A549 cells and H322 cells, respectively. ***P < 0.001