Fig. 2

SNHG22 acted as miR-324-3p sponge in TNBC. a The distribution of SNHG22 in MDA-MB-231 and MDA-MB-468 cells was detected through subcellular fractionation assay. b RIP assay was implemented to verify whether SNHG22 could exist in RISCs. c The possible miRNAs interacted with SNHG22 were predicted from ENCORI under the condition of pan-cancer ≥ 6. d The expression levels of miR-324-3p, miR-200c-3p, miR-331-3p and miR-27a-3p in TNBC cells were tested through qRT-PCR. e, f RIP and RNA pull down experiments were carried out to evaluate the binding situation of miR-324-3p and SNHG22. g The binding sites between miR-324-3p and SNHG22 obtained from ENCORI. h The overexpression efficiency of miR-324-3p was tested by qRT-PCR. i Luciferase reporter experiment was utilized to prove the interaction of miR-324-3p with SNHG22. **P < 0.01