Fig. 3

Knockdown of KIF21B inhibits the proliferation and colony formation abilities of NSCLC cells. H1299 and A549 cells were infected with shRNA-control lentivirus or sh-KIF21B lentivirus, as well as BEAS-2B cells. a qRT-PCR analysis of the expression of KIF21B mRNA in H1299 and A549 cells. b The expression of KIF21B protein in H1299 and A549 cells infected with sh-KIF21B lentivirus was detected by western blot. c qRT-PCR analysis of the expression of KIF21B mRNA in BEAS-2B cells. d The expression of KIF21B protein in BEAS-2B cells was detected by western blot. e–g CCK8 assay was performed to examine the viability of H1299 e, A549 f and BEAS-2B g cells after indicated transfection. h Colony formation abilities of H1299 and A549 cells were assessed by colony formation assay. i Colony formation ability of BEAS-2B cells was assessed by colony formation assay. NC, cells were infected with shRNA-control lentivirus; KIF21B-KD, cells were infected with sh-KIF21B lentivirus (KIF21B-knockdown). Data are presented as the mean ± standard deviation. Results were obtained in three replicates. **P < 0.01