Fig. 6

LIPH overexpression rescued the effect of miR-195-5p on PTC cell progression. TPC-1 and SW579 cells were treated with miR-NC, miR-195-5p, miR-195-5p + pcDNA or miR-195-5p + LIPH. a, b LIPH mRNA and protein levels in TPC-1 and SW579 cells were examined by qRT-PCR assay and western blot assay. c The colony formation of TPC-1 and SW579 cells was determined by colony formation assay. d, e The proliferation of TPC-1 and SW579 cells was evaluated by MTT assay. f The protein level of Ki67 was measured by western blot assay. g The apoptosis of TPC-1 and SW579 cells was assessed using flow cytometry analysis. h, i The migration and invasion of TPC-1 and SW579 cells were measured through transwell assay. j, k The protein levels of Twist1 and E-cadherin in TPC-1 and SW579 cells were detected via western blot assay. *P < 0.05. Each bar represents mean ± SD