Fig. 2

LINC00261 overexpression suppressed the growth of LC cells. a, b The efficiency of LINC00261 overexpression in A549 (a) and SPC-A1 (b) was measured by qRT-PCR, 72 h after infection. (c, d) The viabilities of A549 (c) and SPC-A1 (d) cells after infection for 24 h, 48 h and 72 h were measured by CCK-8 assay. (e–g) The colony formation abilities of A549 (f) and SPC-A1 (g) cells after infection were determined by colony formation assay. (h–k) Cells were harvested 2 weeks after infection and the levels of Ki-67, PCNA in A549 (h–i) and SPC-A1 (j, k) cells were determined by Western blot. GAPDH served as an internal control. ^*P < 0.05, ^**P < 0.001 vs. blank, ^#P < 0.05, ^##P < 0.001 vs. NC. Blank: untreated cells. NC: pCDH-CMV empty plasmid. LINC00261: pCDH-CMV- LINC00261 overexpression plasmid. All experiments repeated independently in triplicate