Fig. 8

MiR-1269a promoted the growth of LC cells through targeting FOXO1. a, b The level of FOXO1 in A549 (a) and SPC-A1 (b) cells was measured by qRT-PCR. c, d The viabilities of A549 (c) and SPC-A1 (d) cells after infection were measured by CCK-8 assay. e–g The colony formation abilities of A549 (f) and SPC-A1 (g) cells after infection were determined by colony formation assay. ^*P < 0.05, ^**P < 0.001, vs Vector. ^#P < 0.05, ^##P < 0.05 vs. FOXO1. ^{^}P < 0.05, ^{^^}P < 0.05 vs. mimic. Vector: pCDH-CMV empty plasmid. FOXO1: pCDH-CMV-FOXO1 overexpression plasmid. Mimic: miR-1269a mimic. FOXO1 + mimic: pCDH-CMV-FOXO1 and miR-1269a mimic. All experiments repeated independently in triplicate