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Fig. 7 | Cancer Cell International

Fig. 7

From: Arsenic trioxide potentiates Gilteritinib-induced apoptosis in FLT3-ITD positive leukemic cells via IRE1a-JNK-mediated endoplasmic reticulum stress

Fig. 7

Addition of ATO to Gilteritinib activates IRE1a-JNK pathway to induce apoptosis. ad MV4-11 and MOLM13 cells were treated with Gilteritinib (5 nM) for 48 h and protein levels of FLT3 were determined by western blot. Data are shown as the mean ± SD. *P < 0.05, ***P < 0.001. e, f MV4-11 cells were treated with Gilteritinib (2.5 nM or 5 nM) for 48 h and protein levels of GRP94, PERK, ATF6 and IRE1a were determined by western blot. Data are shown as the mean ± SD. *P < 0.05, **P < 0.01. g, h MV4-11 cells were treated with Gilteritinib (2.5 nM or 5 nM) for 48 h and protein levels of JNK and P-JNK were determined by western blot. Data are shown as the mean ± SD. **P < 0.01. i, j MV4-11 and MOLM13 cells were treated with Gilteritinib (2.5 nM) and/or ATO (0.5 µM) for 48 h and protein levels of IRE1a, JNK and P-JNK were determined by western blot. Data are shown as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. P-JNK phosphorylated-JNK

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