Table 3 Circulating miRNA(s) in patients suffering from osteosarcoma

miR-9^Om

Increased levels of miR-9 were found to be related to higher TNM stage, distant metastasis and large tumour size; as well as poor S

Fei et al. [233]

miR-17^Om

Increased level was detected in OS patients, where it was linked to poor S; Serum miR-17 levels was reported to be linked to tensin homolog (PTEN) expression and tissue phosphatase

Li et al. [234]

miR-24^Om

Increased serum and tissue miR-24 levels were detected in OS patients

Sun et al. [235]

miR-27a^Om

Higher miR-27a levels was detected to be linked to higher clinical stage, and distant metastasis; Higher miR-27a levels was found to be correlated with poor response to chemotherapy, and was capable of differentiating OS from HC; it serves as an independent prognostic marker of unfavourable survival

Tang et al. [236]

miR-34b^T

Decreased plasma level and low tissue expression of miR-34b were detected, lower level has been found in metastatic patients, it was considered as circulating tumour suppressor miRNA

Tian et al. [58]

miR-25-3p^Om

miR-25-3p level was increased in OS patients; its increase was linked to poor PFS, and it was capable of differentiating OS from healthy control.

Fujiwara et al. [63]

miR-29 family^Om

Higher miR-29a/b/c levels were detected to be associated with OS in evaluated patients; this markers serves as independent prognostic factors of unfavourable survival

Hong et al. [237]

miR-21,

miR-143,

miR-199a-3p^Om

MiR-21 levels increased in OS, whereas miR-143 and miR-199a-3p levels were decreased in OS

Decreased levels of MiR-21 and miR-143 were found to be linked to metastasis and histological subtype; Low level of miR-199-3p was linked to histological subtype

Increased miR-21 was found in the blood, where its high expression was linked to higher Enneking stage and chemotherapeutic resistance

Ouyang et al. [59]; Yuan et al. [60]

miR-95-3p^Ts

Decreased serum level of miR-95-3p was indicated to be linked to clinical stage, metastasis and chemotherapy response. It was considered as circulating tumour suppressor miRNA

Niu et al. [238]

miR-125b^Om

Decreased level of miR-125b was linked to advanced tumour stages

Furthermore, it was capable of differentiating chemotherapy-resistant patients from chemotherapy-sensitive

Luo et al. [53]

miR-133b

miR-206^Ts

MiR-133b and miR-206 downregulation were found to be related to advanced tumour grade, metastasis and recurrence in OS patients’ sera, as well as poor response to chemotherapy in patients. Decreased levels of both miRNAs is attributed to 18 months’ survival time, which is indicated to be a shorter survival in comparison with the mean 24 months survival time in patients with decreased level of only one miRNA; miR-133b and miR-206 may present opportunity as non-invasive biomarker for diagnosis and prognosis of OS

Zhang et al. [239]

miR-148a^Om

Increased expression of circulating miR-148a was linked to increased tumour size and distant metastasis and a negative association with five-year survival in OS patients, where it was revealed to be an independent prognostic factor of unfavourable survival;

As a matter of fact, 148a has been suggested to be a vindicator marker for

progressive phenotype, and a novel diagnostic biomarker

in the peripheral blood for determining poor prognosis in patients suffering from

OS

Ma et al. [240]

miR-152^Ts

Lower serum and tissue levels of miR-152 levels were linked to

Enneking and metastasis in OS patients; decreased level revealed to be capable of differentiating OS from HC, and serves as an independent prognostic factor for unfavourable survival

Wang et al. [241]

miR-196a,

miR-196b^Om

Increased levels of tissue and serum miR-196a and miR-196b were detected;

Higher serum miR-196a and miR-196b and their co-expressions were linked to advanced tumour grade, recurrence and metastasis status in OS patients; expression levels of both MiRs were related to unfavourable survival

Zhang et al. [242]

miR-195-5p,

miR-199a-3p, miR-320a, miR-374a-5p^Om

MiRs levels were elevated in OS patients and their downregulation were found in

postoperative samples; MiR-195-5p and miR-199a-3p were found to be linked to metastasis status, whereas miR-199a-3p and miR-320a levels were related to histological subtype

Lian et al. [62]

miR-199a-5p^Om

Increased levels of miR-199a-5p levels were detected in OS patients; its decreased level were found in postoperative samples; MiR-199-5p was capable of differentiating OS from healthy control

Zhou et al. [243]

miR-221^Om

Increased level of miR-221 in OS patients, and its tissue and serum levels was found to be prognostic factor of unfavourable survival; MiR-221 was found to be capable of differentiating OS from HC

Yang et al. [244]

miR-223^Ts

Decreased level of miR-223 was linked to advanced clinical stage and distant metastasis

Dong et al. [245]

miR-300^Om

Increased tissue and serum miR-300 levels were detected in OS patients; higher clinical stage and distant metastasis were found to be linked to increased level of miR-300 levels; serum levels found to be reduced in OS patients after curative surgery; serum miR-300 was suggested as an independent prognostic marker of unfavourable survival

Liu et al. [246]

miR-326^Ts

Lower serum and tissue levels of miR-326 levels were detected in OS patients; it serves as circulating tumour suppressor miRNA; MiR-326 was capable of differentiating OS from HC;

Decreased serum miR-326 levels were linked to higher clinical stage and distant metastasis, whereas its decrease tissue level was related to distant metastasis; its lower serum level was suggested to be an independent prognostic factor of unfavourable survival

Cao et al. [247]

miR-497^Ts

A circulating tumour suppressor miRNA; lower miR-497 levels was found to be liked to response to chemotherapy, and clinical stage, distant metastasis

Pang et al. [248]