Skip to main content
Fig. 2 | Cancer Cell International

Fig. 2

From: RETRACTED ARTICLE: Circ-DENND4C up-regulates TCF4 expression to modulate hepatocellular carcinoma cell proliferation and apoptosis via activating Wnt/β-catenin signal pathway

Fig. 2

Activated Wnt/β-catenin pathway rescued the effects of circ-DENND4C suppression on HCC cells. a Activators of different signaling pathways were used in immunofluorescence staining assay. b Western blot detected Wnt pathway-associated proteins upon circ-DENND4C depletion. c, d The distribution of β-catenin in cells transfected with sh-NC or sh-circ-DENND4C#1 was determined by western blot analysis and immunofluorescence assay (scale bar = 30 μm). e Colony formation assay detected proliferation of HCC cells transfected with sh-NC, sh-circ-DENND4C#1 or sh-circ-DENND4C#1+LiCl. f Flow cytometry assessed cell cycle in sh-NC, sh-circ-DENND4C#1 or sh-circ-DENND4C#1+LiCl group. g TUNEL assay (scale bar = 100 μm) revealed HCC cell apoptosis with transfection of sh-NC, sh-circ-DENND4C#1 or sh-circ-DENND4C#1+LiCl. h Cyclin D1, CDK4, Bcl-2 and Bax protein levels in each group were confirmed by western blot assay. I. Transwell assay (scale bar = 100 μm) detected cell invasion in HCC cells transfected with sh-NC, sh-circ-DENND4C#1 and sh-circ-DENND4C#1+LiCl. J-K. qRT-PCR and western blot detected protein levels of stemness biomarkers in HCC cells transfected with sh-NC, sh-circ-DENND4C#1 and sh-circ-DENND4C#1+LiCl. *P < 0.05, **P < 0.01

Back to article page

Cancer Cell International

ISSN: 1475-2867

Contact us