Fig. 4

MiR-195-5p targeted TCF4 and negatively modulated TCF4. a qRT-PCR was conducted to examine TCF4 expression in HCC cells and normal THLE-3 cells. b RIP assay verified the interaction between TCF4 and miR-195-5p in HepG2 and Huh7 cells. c Binding sites between TCF4 and miR-195-5p was predicted and the mutant sequences were constructed. d RNA pull down assay detected the interaction between TCF4 and miR-195-5p. e Knockdown efficiency of miR-195-5p was verified by qRT-PCR analysis. f Luciferase reporter assay detected luciferase activity of TCF4-wt/mut reporters under miR-195-5p overexpression or depletion. G-H. Effect of miR-195-5p overexpression or circ-DENND4C depletion on TCF4 mRNA and protein expressions was examined through qRT-PCR and western blot. **P < 0.01