Fig. 1
From: Palbociclib treatment alters nucleotide biosynthesis and glutamine dependency in A549 cells
Palbociclib does not alter glucose uptake or glycolytic function in A549 cells. a Glucose uptake assessed by intracellular 2-[14C]-deoxyglucose in A549 cells following knockdown of RB and 48-h treatment with 1 μM palbociclib. b Glycolysis stress test measured in A549 cells following knockdown of RB and 48-h treatment with 1 μM palbociclib using a Seahorse XFe96 Analyzer. c–f ECAR assessment of c glycolytic capacity, d glycolytic reserve, e glycolysis, and f non-glycolytic acidification. Values represent mean ± SD, analyzed by two-way ANOVA with Sidak’s post hoc multiple comparisons (n = 3, independent experiments)