Fig. 6

Palbociclib increases glutamine dependency and sensitizes A549 cells to glutaminase inhibition. a Glucose, b fatty acid, and c glutamine capacity, and d glucose, e fatty acid, and f glutamine dependency measured by the Mito Fuel Flex assay in A549 cells following 48-h treatment with 1 μM palbociclib. For (a–f), values represent mean ± SD, analyzed by unpaired student’s t-test (n = 3, independent experiments). Statistical significances between each group are as follows: *p < 0.05 and **p < 0.01. g qPCR analysis of glutaminase (GLS) following knockdown of RB and 48-h treatment with 1 μM palbociclib. h Cell proliferation following knockdown of RB and 48-h treatment with 1 μM palbociclib and/or 25 nM CB-839. For (g, h), values represent mean ± SD, analyzed by two-way ANOVA with Sidak’s post hoc multiple comparisons (n = 3, independent experiments). Statistical significances between each group are as follows: ***p < 0.001