Fig. 2
CircDONSON knockdown inhibits DDP resistance of GC cells in vitro. AGS/DDP and HGC-27/DDP cells were transfected with si-NC or si-circDONSON. After transfection, a qRT-PCR analysis of circDONSON expression in AGS/DDP and HGC-27/DDP cells; b CCK-8 assay of the viability and IC50 value of AGS/DDP and HGC-27/DDP cells after exposure to a series dose of DDP (0.125, 0.25, 0.5, 1, 2, 4, or 8 µM); c colony formation assay of the colony-forming ability of AGS/DDP and HGC-27/DDP cells under 1 µM DDP treatment; d flow cytometry of the apoptosis of AGS/DDP and HGC-27/DDP cells under 1 µM DDP treatment; e western blot analysis of Cyclin D1 and p27 levels in AGS/DDP and HGC-27/DDP cells. n = 3, *P < 0.05