Fig. 4

MCM3AP-AS1 targeted miR-876-5p. a Online bioinformatics tools including LncBase and StarBase were used to screen the candidate downstream miRNAs of MCM3AP-AS1. b qRT-PCR was used to detect the expression level of miR-876-5p in PCa tissues and adjacent normal tissues. c The expression of miR-876-5p in normal prostate epithelial cell line and PCa cancer cell line was detected by qRT-PCR. d The correlation between MCM3AP-AS1 and miR-876-5p in PCa tissues was analyzed. e, f The targeting relationship (e) between MCM3AP-AS1 and miR-876-5p was validated by dual luciferase reporter assay (f). g, h After down-regulating the levels of MCM3AP-AS1 in PC-3 (g) and LNCaP (h) cells, the expression of miR-876-5p was observed by qRT-PCR. P < 0.05, P < 0.01, and P < 0.001 were denoted by *, **, and *** respectively