Fig. 4

LINC00242 knockdown retards HGC27 cell viability, migration and invasion, and tube formation of HBMVECs by regulating miR-141 and FOXC1. HGC27 cells were treated with si-LINC00242 alone or with miR-141 inhibitor or oe-FOXC1. a HGC27 cell viability was measured by CCK-8 assay. b Representative view of HGC27 cells invading from Matrigel-coated chambers into lower ones and statistics of invading cells. c Representative view of HGC27 cells migrating from upper transwell chambers into lower ones and statistics of migrating cells. d Representative Western blots of MMP-2, MMP-9, N-cadherin, Vimentin and their quantitation analyses in HGC27 cells. eIn vitro tube formation of HBMVECs in HGC27 cells. Results are presented as mean ± S.D. of three technical replicates. * (compared with si-LINC00242 + inhibitor NC) and # (compared with si-LINC00242 + oe NC) indicate p < 0.05 by unpaired t-test (panel b–e) or repeated measurements ANOVA with Bonferroni corrections (panel a)