Fig. 7

MiR-495-3p played its function via regulation of TRIP13 expression in PCa cells. a Protein level of TRIP13 in 22Rv1 and LNCaP cells was assessed using western blot analysis. a–j 22Rv1 and LNCaP cells were transfected with miR-NC, miR-495-3p, miR-495-3p + pcDNA or miR-495-3p + pcDNA-TRIP13. b, c MTT assay analysis of cell proliferation capacity in 22Rv1 and LNCaP cells. d Flow cytometry assay was applied to detect the apoptotic rate of 22Rv1 and LNCaP cells. e, f The migration and invasion abilities of 22Rv1 and LNCaP cells were evaluated with transwell assay. g–j The levels of Bax, Cleaved-casp-3 and Bcl-2 in 22Rv1 and LNCaP cells were analyzed through western blot analysis. The experiments were performed in triplicate. *P < 0.01 and ***P < 0.001