Fig. 8

NORAD bound to miR-495-3p to regulate TRIP13 expression. a The expression of TRIP13 protein in 22Rv1 and LNCaP cells transfected with si-NC, si-NORAD, si-NORAD + anti-miR-NC or si-NORAD + anti-miR-495-3p was evaluated with western blot analysis. b RNA pull-down assay was employed to verify the relationship between NORAD and TRIP13.  c Pearson’s correlation analysis revealed the correlation  between NORAD and TRIP13 expression in PCa tissues. d Western blot analysis was conducted to assess TRIP13 protein level in 22Rv1 and LNCaP cells. d-m 22Rv1 and LNCaP cells were transfected with si-NC, si-NORAD, si-NORAD + pcDNA or si-NORAD + pcDNA-TRIP13. e, f The proliferation of 22Rv1 and LNCaP cells was detected with MTT assay. g The apoptosis of 22Rv1 and LNCaP cells was analyzed via flow cytometry assay. h, i The migration and invasion capacities of 22Rv1 and LNCaP cells were assessed through transwell assay. j–m Western blot analysis was executed to evaluate the levels of Bax, Cleaved-casp-3 and Bcl-2 protein in 22Rv1 and LNCaP cells. The experiments were performed in triplicate. *P < 0.05, **P < 0.01, and ***P < 0.001