Table 1 The sequence for PCR primer sequences and ANRIL shRNA and control shRNA
Gene | Sequence |
|---|---|
ANRIL | F, 5′-CAACATCCACCACTGGATCTTAACA-3′ |
R, 5′-AGCTTCGTATCCCCAATGAGATACA-3′ | |
miR-7-5p | F, 5′-AAAACTGCTGCCAAAACCAC-3′ |
R, 5′-GCTGCATTTTACAGCGACCAA-3′ | |
U6 | F, 5′-CTCGCTTCGGCAGCACATATACT-3′ |
R, 5′-ACGCTTCACGAATTTGCGTGTC-3′ | |
TCF4 | F, 5′-CTTCCTCCAAACCAGCAACC-3′ |
R, 5′-CCCAACATTCCTGCATAGCC-3′ | |
GAPDH | F, 5′-CGGAGTCAACGGATTTGGTCGTAT-3′ |
R:5′-AGCCTTCTCCATGGTGGTGAAGAC-3′ | |
Scramble shRNA sequence | TCCTAAGGTTAAGTCGCCCTC |
ANRIL shRNA sequence | GGUCAUCUCAUUGCUCUAU |