Fig. 3

MiR-449b-5p potentially interacts with both LINC00667 and YY1. a qRT-PCR analysis of YY1 expression after transfected with LINC0067-specific shRNAs for 48 h. N = 3 in each group. b Promoter luciferase reporter assay was conducted to measure the luciferase activity of YY1 promoter vector in LINC00667-silenced cells. N = 3 in each group. c FISH assay revealed the localization of LINC00667 in CRC cells. Blue: DAPI, green: LINC00667. N = 3 in each group. Scale bar = 100 μm. d The ratio of cytoplasm to nucleus was detected by subcellular fractionation assay, Results were analyzed by qRT-PCR. N = 3 in each group. e Using starBase, DIANA and TargetScan tools, eight miRNAs were predicted to potentially bind to both LINC00667 and YY1. f The binding affinity of eight miRNAs with YY1 was explored by luciferase reporter assays. n = 3 in each group. g The expression of miR-449b-5p in four CRC cells and one colon epithelial cell line (FHC) was measured by qRT-PCR. N = 3 in each group. h MiR-449b-5p expression in two CRC cells transfected with miR-449b-5p mimics or NC mimics for 48 h. N = 3 in each group. i The levels of YY1 mRNA and protein were measured in HCT116 and LoVo cells transfected with miR-449b-5p mimics or NC mimics for 48 h. N = 3 in each group. ^*P < 0.05, ^**P < 0.01 compared to control group