Fig. 2
From: SUV39H1-DNMT3A-mediated epigenetic regulation of Tim-3 and galectin-9 in the cervical cancer
Methylation status in the promoter regions of HAVCR2 and LGALS9 in cervical cancer cells. a Detection of HAVCR2 and LGALS9 methylation status by MS-PCR in SiHa, Hela and C33A cells; (M methylated, U unmethylated). b Tim-3 and galectin-9 expressed in SiHa, HeLa and C33A cells detected by western blotting. c, d Relative expression of HAVCR2 and LGALS9 mRNA in SiHa, HeLa and C33A cells after treatment with different concentrations of 5-Aza-CdR. e Representative immunofluorescence staining of Tim-3 and galectin-9 expression in SiHa and HeLa cells. *P < 0.05, **P < 0.01