Fig. 1

MRTF-A has a positive role in the regulation of miR-155 expression in GC cells. MRTF-A over-expression upregulated miR-155 and MRTF-A RNA levels in SGC-7901 and MGC-803 cells (a, g). MRTF-A over-expression increased MRTF-A protein levels in SGC-7901 and MGC-803 cells (c, e). a, c, e, g MRTF-A encoding plasmids were transfected into gastric cancer cells; pcDNA3.1 was the vector control. Forty-eight hours after transfection, mRNA levels were determined by RT-qPCR and protein levels were determined by Western-blot. GAPDH was used as a loading control. MRTF-A depletion with siRNA reduced miR-155 and MRTF-A RNA levels in SGC-7901 and MGC-803 cells (b, h). MRTF-A depletion with siRNA suppressed MRTF-A protein expression in SGC-7901 and MGC-803 cells (d, f). b, d, f, h Cells were transfected with siMRTF-A duplexes. mRNA and protein levels were determined 48 h after transfection. i MRTF-A over-expression elevated miR-155 promoter activity in SGC-7901 gastric cancer cells. j siMRTF-A reduced miR-155 promoter activity in SGC-7901 gastric cancer cells. Data are shown as the mean ± S.E.M, based on three to six independent experiments. **, p < 0.01