Fig. 3
From: Circular RNA hsa_circ_0005909 modulates osteosarcoma progression via the miR-936/HMGB1 axis
Circ_0005909 targeted miR-936 in OS cells. a The binding sites of miR-936 in circ_0005909 were predicted by the Circinteractome database. b, c The luciferase intensities of the circ_0005909-WT and circ_0005909-MUT reporter vectors in HOS and U2OS cells transfected with miR-936 or miR-NC were determined via dual-luciferase reporter assay. d The relationship between miR-936 and circ_0005909 was further verified through RNA pull-down assay. e Expression of miR-936 in circ_0005909-suppressed HOS and U2OS cells was examined by qRT-PCR. f, g Expression of miR-936 in OS cells (f) and tissues (g) was analyzed via qRT-PCR. h The correlation between miR-936 and circ_0005909 in OS tissues was assessed with Pearson’s correlation analysis. *P < 0.05, **P < 0.01, and ***P < 0.001