Fig. 5

MiR-936 targeted HMGB1 in OS cells. a The binding sites between the 3′UTR of HMGB1 and miR-936 were predicted via Targetscan database. b, c Dual-luciferase reporter assay was carried out for the evaluation of the luciferase activities of the luciferase reporter vectors HMGB1 3′UTR-WT and HMGB1 3′UTR-MUT in HOS and U2OS cells transfected with miR-936 or miR-NC, respectively. d, e Effects of miR-936 upregulation on HMGB1 mRNA and protein expression of HOS and U2OS cells were examined via qRT-PCR or western blot analysis. f, g Influence of miR-936 suppression on HMGB1 mRNA and protein expression of HOS and U2OS cells caused by circ_0005909 downregulation was surveyed by qRT-PCR or western blot analysis. *P < 0.05 and **P < 0.01