Fig. 3
RT-qPCR and western blotting of the expression level of key genes in RCC cell lines. a–f RT-qPCR detection of mRNA expression of SUCLG1, PCK2, GLDC, SLC12A1, ATP1A1, PDHA1 in seven RCC cell lines. g Key genes mRNA expression level in eight paired kidney tumor tissues. h SUCLG1, PCK2, GLDC protein expression level in eight paired kidney tumor tissues (T) and their adjacent normal tissues (ANT). i The values of the band intensity represent the densitometric estimation of each band normalised by β-actin in (H). (*p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.001)