Fig. 4

Cyclin H regulates the proliferation and cell cycle of ovarian cancer cells. a mRNA level of cyclin H in vector and cyclin H shRNA transfected ovarian cancer HO8910 cells. b The protein expression of cyclin H was detected by Western blot. c Proliferation difference between control and cyclin H silencing HO8910 cells. PI staining was performed to measure the percentage of cells in each cell cycle phase using Modfit software (d and e). f Quantification of the percentage cells in each cell cycle phase after transfection with cyclin H shRNA. g Expression of cyclin H, CDK7, MAT1, p-CDK2, and CDK2 in HO8910 cells after serum deprivation and refeeding. Serum-starved HO8910 cells were cultured in serum-containing medium for 4, 8, 12, 24, and 48 h, and cell lysates were analyzed by western blot. h Relative level of p-CDK2 was normalized to the total level of CDK2 at each time point. * P < 0.05, **P < 0.01, ***P < 0.001